Purification step

Volume

(mL)

Protein

Enzyme Activity

% Yield

Concentration (mg∙mL⁻¹)

Total (mg)

Specific activity

(Units mg⁻¹ × 10³)

Total Activity (Units)

Fresh milk

Bovine Caprine Ovine

1, 775 2,800 2,800

26.82 ± 3.56 23.63 ± 3.17 50.68 ± 3.46

47,000.0 66,000.0 142,000.0

1.0 ± 0.1 0.5 ± 0.2 0.3 ± 0.2

47.00 35.00 42.60

100 100 100

n-butanol extraction

Bovine Caprine Ovine

62 222 200

6.89 ± 0.65 8.24 ± 0.29 12.17 ± 0.13

427.8 1820.4 2440.0

26.0 ± 0.6 1.0 ± 0.4 3.0 ± 0.3

11.10 1.80 7.30

24 5

17

Ammonium sulphate (20%)

Bovine Caprine Ovine

11 27 16

10.14 ± 0.45 8.19 ± 0.50 7.50 ± 0.35

111.1 139.3 120.0

43.0 ± 2.0 13.0 ± 1.0 4.0 ± 0.2

4.78 1.81 0.48

10 5

11

Heparin column

Bovine Caprine Ovine

5 4 5

1.45 ± 0.09 4.49 ± 0.21 3.61 ± 0.09

7.2 18.0 18.0

131.0 ± 6.0 26.0 ± 2.0 6.0 ± 1.0

0.94 0.47 0.11

2 1 0.3